Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil.

A crescente notificação de leishmaniose tegumentar no Estado do Paraná requer a busca de explicações para o esclarecimento da epidemiologia desta doença e dos meios para o seu controle. Realizou-se um inquérito sorológico para investigar leishmaniose tegumentar em cães de seis propriedades agrícolas da Companhia Melhoramentos Norte do Paraná, situadas nos municípios de Jussara e Terra Boa, no Noroeste do Estado do Paraná. Foram investigados 132 cães, dos quais 24 (18,2%) apresentaram títulos significativos de anticorpos através da técnica de Imunofluorescência Indireta (IFI). De seis cães apresentando lesões foram feitas lâminas de esfregaço por aposição, as quais foram negativas para Leishmania sp. à microscopia direta.

Increased reporting of mucocutaneous leishmaniasis in the State of Paraná points to the need for information on both this disease's epidemiology and pertinent control measures. A serological survey was thus performed, with indirect immunofluorescence (IIF) for canine leishmaniasis in farm operations belonging to the Companhia Melhoramentos Norte do Paraná, in Jussara and Terra Boa counties, in northwestern Paraná. IIF was performed on 132 dogs, of which 24 (18.2%) had significant titers ( > or = 40). Imprints of six dogs with lesions were made and all were negative for Leishmania sp.

A crescente notificação de leishmaniose tegumentar no Estado do Paraná requer a busca de explicações para o esclarecimento da epidemiologia desta doença e dos meios para o seu controle. Realizou-se um inquérito sorológico para investigar leishmaniose tegumentar em cães de seis propriedades agrícolas da Companhia Melhoramentos Norte do Paraná, situadas nos municípios de Jussara e Terra Boa, no Noroeste do Estado do Paraná. Foram investigados 132 cães, dos quais 24 (18,2%) apresentaram títulos significativos de anticorpos através da técnica de Imunofluorescência Indireta (IFI). De seis cães apresentando lesões foram feitas lâminas de esfregaço por aposição, as quais foram negativas para Leishmania sp. à microscopia direta.

Increased reporting of mucocutaneous leishmaniasis in the State of Paraná points to the need for information on both this disease's epidemiology and pertinent control measures. A serological survey was thus performed, with indirect immunofluorescence (IIF) for canine leishmaniasis in farm operations belonging to the Companhia Melhoramentos Norte do Paraná, in Jussara and Terra Boa counties, in northwestern Paraná. IIF was performed on 132 dogs, of which 24 (18.2%) had significant titers ( > or = 40). Imprints of six dogs with lesions were made and all were negative for Leishmania sp.